BioFSharp
RNA-Seq data normalization
RNA-Seq is a high-throughput transcriptomics technique, that quantifies RNA molecules in a biological sample. RNA-Seq provides a view of the whole transcriptome and allows to look at gene-expression, post-transcriptonal modifications and look at different populations of RNA. When dealing with RNA-sequencing data, normalization is needed to correct technical biases. RPKM and TPM are two metrics that normalize for gene length and sequencing depth. RNA-Sequencing data needs to be normalized for gene length, because longer genes show greater read counts when expressed at the same level and for sequencing depth, as deeper sequencing depth produces more read counts per gene.
RPKM (Reads per kilobase million)
RPKM (Reads per kilobase million) normalization at first determines a scaling factor, by calculating the sum of all reads in a sample and dividing that number by 1,000,000. That scaling factor is used to calculate RPM (Reads per million), by dividing the read counts for each sample with it, normalizing for sequencing depth. To get RPKM and normalize for gene length, RPM values are divided by genelength in kilobases. RPKM is calculated by \[RPKM = 10^9 * \frac {\text {Reads mapped to transcript}} {\text {Total reads * Transcript length}}\]
The formula is applied by using the RNASeq.rpkms function.
The following dataset will be used as an example for both normalizations.
open BioFSharp.Stats
let rawDataGeneID = seq {"g01";"g02";"g03";"g04";"g05"}
let rawDataGeneLength = seq {150.; 500.; 1500.; 500.; 1700.}
let rawDataGeneCount = seq {16.; 53.; 156.; 52.; 180.}
let rawData = Seq.map3 (fun id gl gc -> RNASeq.RNASeqInput.Create id gl gc) rawDataGeneID rawDataGeneLength rawDataGeneCount
// rpkm normalization
open BioFSharp.Stats
let rpkmData = RNASeq.rpkms rawData
TPM (Transcripts per kilobase million)
What differentiates TPM (Transcripts per kilobase million) from RPKM is the order of operations. To calculate TPM values, data gets normalized for gene length first. This is achieved by calculating RPK values (reads per kilobase), by dividing the read counts by genelength in kilobases. The sum of all RPK values is divided by 1,000,000, to get a scaling factor. Finally, TPM values are calculated by dividing the RPK values by the scaling factor, also normalizing for sequencing depth. By normalizing for gene length first, the sum of all samples is always 1,000,000, making comparisons of proportions easier. TPM is calculated by \[TPM = 10^6 * \frac {\text{Reads mapped to transcript / Transcript length}} {\text {Sum(reads mapped to transcript / Transcript length)}}\]
The formula is applied by using the RNASeq.tpms function.
// tpm normalization
open BioFSharp.Stats
let tpmData = RNASeq.tpms rawData
The effects of both normalizations becomes apparent when comparing the relation of the samples
// visualization of Raw Data, RPKM & TPM
let rawDataCounts =
rawData
|> Seq.map (fun input -> input.GeneCount)
let rawDataKeys =
rawData
|> Seq.map (fun input -> input.GeneID)
let rawDataLength =
rawData
|> Seq.map (fun input -> input.GeneLength)
let rpkmDataCounts =
rpkmData
|> Seq.map (fun rpkmData -> rpkmData.NormalizedCount)
let rpkmDataKeys =
rpkmData
|> Seq.map (fun rpkmData -> rpkmData.GeneID)
let tpmDataCounts =
tpmData
|> Seq.map (fun tpmData -> tpmData.NormalizedCount)
let tpmDataKeys =
tpmData
|> Seq.map (fun tpmData -> tpmData.GeneID)
[
Chart.Column(values = rawDataLength, Keys = rawDataKeys, Name = "Gene Length")
|> Chart.withXAxisStyle(TitleText = "gene ID")
|> Chart.withYAxisStyle(TitleText = "gene Length", TitleStandoff = 2, MinMax = (0., 1800.))
Chart.Column(values = rawDataCounts, Keys = rawDataKeys, Name = "raw data counts")
|> Chart.withXAxisStyle(TitleText = "gene ID")
|> Chart.withYAxisStyle(TitleText = "Read Counts", TitleStandoff = 2, MinMax = (0.,200.))
Chart.Column(values = rpkmDataCounts, Keys = rpkmDataKeys, Name = "RPKM")
|> Chart.withXAxisStyle(TitleText = "gene ID")
|> Chart.withYAxisStyle(TitleText = "Read Counts", TitleStandoff = 2, MinMax = (0.,260000.))
Chart.Column(values = tpmDataCounts, Keys = tpmDataKeys, Name = "TPM")
|> Chart.withXAxisStyle(TitleText = "gene ID")
|> Chart.withYAxisStyle(TitleText = "Read Counts", TitleStandoff = 2, MinMax = (0.,260000.))
]
|> Chart.Grid(nRows = 1, nCols = 4, XGap = 0.35)
|> Chart.withSize (1000)
These graphs show how RPKM and TPM correct for technical biases, especially gene length, as the three shorter genes (g01, g02 & g04) are no longer underrepresented.
Sources: RNA-Seqblog
namespace Plotly
namespace Plotly.NET
namespace Plotly.NET.Interactive
namespace BioFSharp
namespace BioFSharp.Stats
val rawDataGeneID: string seq
Multiple items
val seq: sequence: 'T seq -> 'T seq
--------------------
type 'T seq = System.Collections.Generic.IEnumerable<'T>
val seq: sequence: 'T seq -> 'T seq
--------------------
type 'T seq = System.Collections.Generic.IEnumerable<'T>
val rawDataGeneLength: float seq
val rawDataGeneCount: float seq
val rawData: RNASeq.RNASeqInput seq
module Seq
from Microsoft.FSharp.Collections
val map3: mapping: ('T1 -> 'T2 -> 'T3 -> 'U) -> source1: 'T1 seq -> source2: 'T2 seq -> source3: 'T3 seq -> 'U seq
val id: string
val gl: float
val gc: float
module RNASeq
from BioFSharp.Stats
<summary> Contains types and functions needed for RNA-Seq normalization </summary>
<summary> Contains types and functions needed for RNA-Seq normalization </summary>
type RNASeqInput =
{
GeneID: string
GeneLength: float
GeneCount: float
}
static member Create: id: string -> gl: float -> gc: float -> RNASeqInput
<summary> Input type for RNA-Seq normalization </summary>
<summary> Input type for RNA-Seq normalization </summary>
static member RNASeq.RNASeqInput.Create: id: string -> gl: float -> gc: float -> RNASeq.RNASeqInput
val rpkmData: RNASeq.NormalizedCounts seq
val rpkms: idLengthAndCounts: RNASeq.RNASeqInput seq -> RNASeq.NormalizedCounts seq
<summary> Returns RPKM normalized data </summary>
<summary> Returns RPKM normalized data </summary>
val tpmData: RNASeq.NormalizedCounts seq
val tpms: idLengthAndCounts: RNASeq.RNASeqInput seq -> RNASeq.NormalizedCounts seq
<summary> Returns TPM normalized data </summary>
<summary> Returns TPM normalized data </summary>
val rawDataCounts: float seq
val map: mapping: ('T -> 'U) -> source: 'T seq -> 'U seq
val input: RNASeq.RNASeqInput
RNASeq.RNASeqInput.GeneCount: float
val rawDataKeys: string seq
RNASeq.RNASeqInput.GeneID: string
val rawDataLength: float seq
RNASeq.RNASeqInput.GeneLength: float
val rpkmDataCounts: float seq
val rpkmData: RNASeq.NormalizedCounts
RNASeq.NormalizedCounts.NormalizedCount: float
val rpkmDataKeys: string seq
RNASeq.NormalizedCounts.GeneID: string
val tpmDataCounts: float seq
val tpmData: RNASeq.NormalizedCounts
val tpmDataKeys: string seq
type Chart =
static member AnnotatedHeatmap: zData: #('a1 seq) seq * annotationText: #(string seq) seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?X: 'a3 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiX: 'a3 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?XGap: int * [<Optional; DefaultParameterValue ((null :> obj))>] ?Y: 'a4 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiY: 'a4 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?YGap: int * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a5 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a5 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorBar: ColorBar * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ReverseScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ZSmooth: SmoothAlg * [<Optional; DefaultParameterValue ((null :> obj))>] ?Transpose: bool * [<Optional; DefaultParameterValue ((false :> obj))>] ?UseWebGL: bool * [<Optional; DefaultParameterValue ((false :> obj))>] ?ReverseYAxis: bool * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a1 :> IConvertible and 'a3 :> IConvertible and 'a4 :> IConvertible and 'a5 :> IConvertible) + 1 overload
static member Area: x: #IConvertible seq * y: #IConvertible seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowMarkers: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextPosition: TextPosition * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: TextPosition seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerSymbol: MarkerSymbol * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiMarkerSymbol: MarkerSymbol seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineWidth: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineDash: DrawingStyle * [<Optional; DefaultParameterValue ((null :> obj))>] ?Line: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?AlignmentGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?OffsetGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?StackGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?Orientation: Orientation * [<Optional; DefaultParameterValue ((null :> obj))>] ?GroupNorm: GroupNorm * [<Optional; DefaultParameterValue ((null :> obj))>] ?FillColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?FillPatternShape: PatternShape * [<Optional; DefaultParameterValue ((null :> obj))>] ?FillPattern: Pattern * [<Optional; DefaultParameterValue ((false :> obj))>] ?UseWebGL: bool * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a2 :> IConvertible) + 1 overload
static member Bar: values: #IConvertible seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Keys: 'a1 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiKeys: 'a1 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerPatternShape: PatternShape * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiMarkerPatternShape: PatternShape seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerPattern: Pattern * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?Base: #IConvertible * [<Optional; DefaultParameterValue ((null :> obj))>] ?Width: 'a4 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiWidth: 'a4 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextPosition: TextPosition * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: TextPosition seq * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a1 :> IConvertible and 'a2 :> IConvertible and 'a4 :> IConvertible) + 1 overload
static member BoxPlot: [<Optional; DefaultParameterValue ((null :> obj))>] ?X: 'a0 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiX: 'a0 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Y: 'a1 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiY: 'a1 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?FillColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?WhiskerWidth: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?BoxPoints: BoxPoints * [<Optional; DefaultParameterValue ((null :> obj))>] ?BoxMean: BoxMean * [<Optional; DefaultParameterValue ((null :> obj))>] ?Jitter: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?PointPos: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Orientation: Orientation * [<Optional; DefaultParameterValue ((null :> obj))>] ?OutlineColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?OutlineWidth: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Outline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?AlignmentGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?OffsetGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?Notched: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?NotchWidth: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?QuartileMethod: QuartileMethod * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a0 :> IConvertible and 'a1 :> IConvertible and 'a2 :> IConvertible) + 2 overloads
static member Bubble: x: #IConvertible seq * y: #IConvertible seq * sizes: int seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextPosition: TextPosition * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: TextPosition seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerSymbol: MarkerSymbol * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiMarkerSymbol: MarkerSymbol seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineWidth: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?LineDash: DrawingStyle * [<Optional; DefaultParameterValue ((null :> obj))>] ?Line: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?AlignmentGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?OffsetGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?StackGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?Orientation: Orientation * [<Optional; DefaultParameterValue ((null :> obj))>] ?GroupNorm: GroupNorm * [<Optional; DefaultParameterValue ((false :> obj))>] ?UseWebGL: bool * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a2 :> IConvertible) + 1 overload
static member Candlestick: ``open`` : #IConvertible seq * high: #IConvertible seq * low: #IConvertible seq * close: #IConvertible seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?X: 'a4 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiX: 'a4 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a5 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a5 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Line: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?IncreasingColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?Increasing: FinanceMarker * [<Optional; DefaultParameterValue ((null :> obj))>] ?DecreasingColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?Decreasing: FinanceMarker * [<Optional; DefaultParameterValue ((null :> obj))>] ?WhiskerWidth: float * [<Optional; DefaultParameterValue ((true :> obj))>] ?ShowXAxisRangeSlider: bool * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a4 :> IConvertible and 'a5 :> IConvertible) + 2 overloads
static member Column: values: #IConvertible seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Keys: 'b seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiKeys: 'b seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'c * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'c seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerPatternShape: PatternShape * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiMarkerPatternShape: PatternShape seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerPattern: Pattern * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?Base: #IConvertible * [<Optional; DefaultParameterValue ((null :> obj))>] ?Width: 'e * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiWidth: 'e seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextPosition: TextPosition * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: TextPosition seq * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'b :> IConvertible and 'c :> IConvertible and 'e :> IConvertible) + 1 overload
static member Contour: zData: #('a1 seq) seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?X: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiX: 'a2 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Y: 'a3 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiY: 'a3 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a4 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a4 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorBar: ColorBar * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ReverseScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Transpose: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContourLineColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContourLineDash: DrawingStyle * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContourLineSmoothing: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContourLine: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContoursColoring: ContourColoring * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContoursOperation: ConstraintOperation * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContoursType: ContourType * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowContourLabels: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ContourLabelFont: Font * [<Optional; DefaultParameterValue ((null :> obj))>] ?Contours: Contours * [<Optional; DefaultParameterValue ((null :> obj))>] ?FillColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?NContours: int * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a1 :> IConvertible and 'a2 :> IConvertible and 'a3 :> IConvertible and 'a4 :> IConvertible)
static member Funnel: x: #IConvertible seq * y: #IConvertible seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Width: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Offset: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextPosition: TextPosition * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: TextPosition seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Orientation: Orientation * [<Optional; DefaultParameterValue ((null :> obj))>] ?AlignmentGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?OffsetGroup: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?Marker: Marker * [<Optional; DefaultParameterValue ((null :> obj))>] ?TextInfo: TextInfo * [<Optional; DefaultParameterValue ((null :> obj))>] ?ConnectorLineColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?ConnectorLineStyle: DrawingStyle * [<Optional; DefaultParameterValue ((null :> obj))>] ?ConnectorFillColor: Color * [<Optional; DefaultParameterValue ((null :> obj))>] ?ConnectorLine: Line * [<Optional; DefaultParameterValue ((null :> obj))>] ?Connector: FunnelConnector * [<Optional; DefaultParameterValue ((null :> obj))>] ?InsideTextFont: Font * [<Optional; DefaultParameterValue ((null :> obj))>] ?OutsideTextFont: Font * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a2 :> IConvertible)
static member Heatmap: zData: #('a1 seq) seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?X: 'a2 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiX: 'a2 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Y: 'a3 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiY: 'a3 seq seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?Name: string * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<Optional; DefaultParameterValue ((null :> obj))>] ?XGap: int * [<Optional; DefaultParameterValue ((null :> obj))>] ?YGap: int * [<Optional; DefaultParameterValue ((null :> obj))>] ?Text: 'a4 * [<Optional; DefaultParameterValue ((null :> obj))>] ?MultiText: 'a4 seq * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorBar: ColorBar * [<Optional; DefaultParameterValue ((null :> obj))>] ?ColorScale: Colorscale * [<Optional; DefaultParameterValue ((null :> obj))>] ?ShowScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ReverseScale: bool * [<Optional; DefaultParameterValue ((null :> obj))>] ?ZSmooth: SmoothAlg * [<Optional; DefaultParameterValue ((null :> obj))>] ?Transpose: bool * [<Optional; DefaultParameterValue ((false :> obj))>] ?UseWebGL: bool * [<Optional; DefaultParameterValue ((false :> obj))>] ?ReverseYAxis: bool * [<Optional; DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart (requires 'a1 :> IConvertible and 'a2 :> IConvertible and 'a3 :> IConvertible and 'a4 :> IConvertible) + 1 overload
...
static member Chart.Column: keysValues: (#System.IConvertible * #System.IConvertible) seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Name: string * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Text: 'a2 * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiText: 'a2 seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: StyleParam.Colorscale * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPatternShape: StyleParam.PatternShape * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiMarkerPatternShape: StyleParam.PatternShape seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPattern: TraceObjects.Pattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Marker: TraceObjects.Marker * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Base: #System.IConvertible * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Width: 'a4 * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiWidth: 'a4 seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TextPosition: StyleParam.TextPosition * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: StyleParam.TextPosition seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart.GenericChart (requires 'a2 :> System.IConvertible and 'a4 :> System.IConvertible)
static member Chart.Column: values: #System.IConvertible seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Keys: 'b seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiKeys: 'b seq seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Name: string * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Text: 'c * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiText: 'c seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: StyleParam.Colorscale * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPatternShape: StyleParam.PatternShape * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiMarkerPatternShape: StyleParam.PatternShape seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPattern: TraceObjects.Pattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Marker: TraceObjects.Marker * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Base: #System.IConvertible * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Width: 'e * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiWidth: 'e seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TextPosition: StyleParam.TextPosition * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: StyleParam.TextPosition seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart.GenericChart (requires 'b :> System.IConvertible and 'c :> System.IConvertible and 'e :> System.IConvertible)
static member Chart.Column: values: #System.IConvertible seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Keys: 'b seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiKeys: 'b seq seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Name: string * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowLegend: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Opacity: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiOpacity: float seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Text: 'c * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiText: 'c seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerColorScale: StyleParam.Colorscale * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerOutline: Line * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPatternShape: StyleParam.PatternShape * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiMarkerPatternShape: StyleParam.PatternShape seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MarkerPattern: TraceObjects.Pattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Marker: TraceObjects.Marker * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Base: #System.IConvertible * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Width: 'e * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiWidth: 'e seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TextPosition: StyleParam.TextPosition * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MultiTextPosition: StyleParam.TextPosition seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((true :> obj))>] ?UseDefaults: bool -> GenericChart.GenericChart (requires 'b :> System.IConvertible and 'c :> System.IConvertible and 'e :> System.IConvertible)
static member Chart.withXAxisStyle: [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleText: string * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleFont: Font * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleStandoff: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Title: Title * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Color: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?AxisType: StyleParam.AxisType * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MinMax: (#System.IConvertible * #System.IConvertible) * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Mirror: StyleParam.Mirror * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowSpikes: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SpikeColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SpikeThickness: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowLine: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?LineColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowGrid: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?GridColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?GridDash: StyleParam.DrawingStyle * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ZeroLine: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ZeroLineColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Anchor: StyleParam.LinearAxisId * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Side: StyleParam.Side * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Overlaying: StyleParam.LinearAxisId * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Domain: (float * float) * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Position: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?CategoryOrder: StyleParam.CategoryOrder * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?CategoryArray: #System.IConvertible seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RangeSlider: LayoutObjects.RangeSlider * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RangeSelector: LayoutObjects.RangeSelector * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?BackgroundColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowBackground: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Id: StyleParam.SubPlotId -> (GenericChart.GenericChart -> GenericChart.GenericChart)
static member Chart.withYAxisStyle: [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleText: string * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleFont: Font * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?TitleStandoff: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Title: Title * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Color: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?AxisType: StyleParam.AxisType * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?MinMax: (#System.IConvertible * #System.IConvertible) * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Mirror: StyleParam.Mirror * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowSpikes: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SpikeColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SpikeThickness: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowLine: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?LineColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowGrid: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?GridColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?GridDash: StyleParam.DrawingStyle * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ZeroLine: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ZeroLineColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Anchor: StyleParam.LinearAxisId * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Side: StyleParam.Side * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Overlaying: StyleParam.LinearAxisId * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?AutoShift: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Shift: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Domain: (float * float) * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Position: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?CategoryOrder: StyleParam.CategoryOrder * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?CategoryArray: #System.IConvertible seq * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RangeSlider: LayoutObjects.RangeSlider * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RangeSelector: LayoutObjects.RangeSelector * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?BackgroundColor: Color * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?ShowBackground: bool * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Id: StyleParam.SubPlotId -> (GenericChart.GenericChart -> GenericChart.GenericChart)
static member Chart.Grid: [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SubPlots: (StyleParam.LinearAxisId * StyleParam.LinearAxisId) array array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RowOrder: StyleParam.LayoutGridRowOrder * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Pattern: StyleParam.LayoutGridPattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Domain: LayoutObjects.Domain * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XSide: StyleParam.LayoutGridXSide * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YSide: StyleParam.LayoutGridYSide -> (#('a1 seq) -> GenericChart.GenericChart) (requires 'a1 :> GenericChart.GenericChart seq)
static member Chart.Grid: nRows: int * nCols: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SubPlots: (StyleParam.LinearAxisId * StyleParam.LinearAxisId) array array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RowOrder: StyleParam.LayoutGridRowOrder * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Pattern: StyleParam.LayoutGridPattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Domain: LayoutObjects.Domain * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XSide: StyleParam.LayoutGridXSide * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YSide: StyleParam.LayoutGridYSide -> (#(GenericChart.GenericChart seq) -> GenericChart.GenericChart)
static member Chart.Grid: nRows: int * nCols: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?SubPlots: (StyleParam.LinearAxisId * StyleParam.LinearAxisId) array array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YAxes: StyleParam.LinearAxisId array * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?RowOrder: StyleParam.LayoutGridRowOrder * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Pattern: StyleParam.LayoutGridPattern * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YGap: float * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Domain: LayoutObjects.Domain * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?XSide: StyleParam.LayoutGridXSide * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?YSide: StyleParam.LayoutGridYSide -> (#(GenericChart.GenericChart seq) -> GenericChart.GenericChart)
static member Chart.withSize: width: float * height: float -> (GenericChart.GenericChart -> GenericChart.GenericChart)
static member Chart.withSize: [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Width: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Height: int -> (GenericChart.GenericChart -> GenericChart.GenericChart)
static member Chart.withSize: [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Width: int * [<System.Runtime.InteropServices.Optional; System.Runtime.InteropServices.DefaultParameterValue ((null :> obj))>] ?Height: int -> (GenericChart.GenericChart -> GenericChart.GenericChart)