!! Currently many parameters are not implemented!

From the Last docs at http://last.cbrc.jp:

LAST finds similar regions between sequences.

LAST can:
 
Handle big sequence data, e.g:

     - Compare two vertebrate genomes

     - Align billions of DNA reads to a genome

Indicate the reliability of each aligned column.

Use sequence quality data properly.

Compare DNA to proteins, with frameshifts.

Compare PSSMs to sequences

Calculate the likelihood of chance similarities between random sequences.

Do split and spliced alignment.

Train alignment parameters for unusual kinds of sequence (e.g. nanopore).