BasicWorkflows
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AligmentPairedEndReadOptions
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AlignmentChimericOptions
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AlignmentEndsProtrusionOptions
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maximum and type of allowed allow protrusion of alignment ends, i.e. start (end) of the +strand mate downstream of the start (end) of the -strand mate
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AlignmentEndsTypeOptions
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type of read ends alignment
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AlignmentGeneralOptions
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AlignmentInsertionFlushOptions
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how to flush ambiguous insertion positions
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AlignmentOrder
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AlignmentParams
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AlignmentScoringOptions
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AlignmentSeedOptions
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AlignmentWindowOptions
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Windows, Anchors, Binning Options
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AnnotationQuantificationModeOptions
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types of quantification requested
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AnnotationQuantificationParams
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AttributeOptions
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desired SAM attributes, in the order desired for the output SAM
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BAMHandlingOptions
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ChimericAlignmentFilterOptions
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different filters for chimeric alignments
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ChimericAlignmentOutputTypeOptions
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ChimericOutJunctionFormatOptions
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formatting type for the Chimeric.out.junction file
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GenomeIndexingOptions
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Genome Indexing Parameters - only used with --runMode genomeGenerate
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GenomeLoad
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mode of shared memory usage for the genome files. Only used with --runMode alignReads.
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GenomeParams
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LimitParams
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MateClippingOptions
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clipping options from either end of the mates
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MatesLengths
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lengths of names,sequences,qualities for both mates are the same / not the same. NotEqual is safe in all situations.
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OutputFilterIntronMotifsOptions
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filter alignment using their motifs
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OutputFilterOptions
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filter the output into main SAM/BAM files
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OutputFilterTypeOptions
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OutputFilteringParams
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OutputFilteringSpliceJunctionsOptions
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OutputFormattingOptions
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OutputModeOptions
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OutputParams
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OutputSTDOptions
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OutputSortingOptions
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type of sorting for the SAM output
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OutputTypeOptions
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OutputWiggleParams
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PrimaryFlagOptions
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which alignments are considered primary - all others will be marked with 0x100 bit in the FLAG
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ReadFilesType
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format of input read files
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ReadIDOptions
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ReadParams
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RemoveDuplicatesTypeOptions
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mark duplicates in the BAM file, for now only works with (i) sorted BAM fed with inputBAMfile, and (ii) for paired-end alignments only
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RunMode
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RunParams
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RunPermission
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SAMSortingOptions
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STARParams
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STARSoloBarcodeReadLengthOptions
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length of the barcode read
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STARSoloCellBarcodeWhiteListOptions
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matching the Cell Barcodes to the WhiteList
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STARSoloCellFilterOptions
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cell filtering type and parameters
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STARSoloFeaturesOptions
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genomic features for which the UMI counts per Cell Barcode are collected
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STARSoloParams
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STARSoloStrandOptions
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strandedness of the solo libraries:
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STARSoloTypeOptions
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type of single-cell RNA-seq
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STARSoloUMIDeDuplicationOptions
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type of UMI deduplication (collapsing) algorithm
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STARSoloUMIFilteringOptions
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SpliceJunctionsDatabaseParams
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SpliceJunctionsDatabaseSaveOptions
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which files to save when sjdb junctions are inserted on the fly at the mapping step
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SpliceJunctionsFilterReadOptions
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which reads to consider for collapsed splice junctions output
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StrandFieldOptions
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Cufflinks-like strand field flag
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TranscriptomeBanOptions
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prohibit various alignment type
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TwoPassMappingParams
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TwoPassModeOptions
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UnmappedReadOptions
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output of unmapped reads in the SAM format
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WASPOutputModeOptions
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WASP allele-specific output type. This is re-implemenation of the original WASP mappability filtering by Bryce van de Geijn, Graham McVicker, Yoav Gilad & Jonathan K Pritchard. Please cite the original WASP paper: Nature Methods 12, 1061�1063 (2015), https://www.nature.com/articles/nmeth.3582 .
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WASPParams
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WiggleNormalizationOptions
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WiggleSignal
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WiggleStrandOptions
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WiggleTypeOptions
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type of signal output, e.g. "bedGraph" OR "bedGraph read1_5p". Requires sorted BAM: --outSAMtype BAM SortedByCoordinate .
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